K. A. Lidke

Universal inverse modelling of point spread functions for SMLM localization and microscope characterization

This paper introduces uiPSF, a toolbox to infer accurate PSF models from microscopy data for improved SMLM imaging and microscope characterization.

Axial de-scanning using remote focusing in the detection arm of light-sheet microscopy

This paper introduces a novel optical design for axial de-scanning in light-sheet microscopy's detection arm, enabling aberration-free, multi-color volumetric imaging without sample translation.

Antigen properties shape organization of FcεRI aggregates to tune mast cell signaling

This study shows that antigen properties like valency and epitope spacing shape FcεRI aggregate structure, influencing mast cell signaling outcomes.

Signal Improved ultra-Fast Light-sheet Microscope (SIFT) for large tissue imaging

The paper introduces an improved light-sheet microscope that increases imaging speed and signal strength for large tissue samples.

Distinct interactions stabilize EGFR dimers and higher-order oligomers in cell membranes

This study investigates how EGFR dimers and higher-order oligomers form and interact in cell membranes using SPT and FRET, revealing the role of the tyrosine kinase domain in oligomerization.

Robust, Fiducial-Free Drift Correction for Super-resolution Imaging

This paper introduces a drift correction method for super-resolution imaging that uses brightfield images and post-processing to correct drift without fiducials or specialized hardware.

Comparing Lifeact and Phalloidin for Super-resolution Imaging of Actin in Fixed Cells

This study compares Lifeact and Phalloidin for super-resolution imaging of actin in fixed cells, finding Lifeact offers comparable resolution with additional benefits.

EGFR transactivates RON to drive oncogenic crosstalk

This study identifies EGFR-dependent phosphorylation of RON and shows direct EGFR-RON interactions drive oncogenic crosstalk, contributing to therapeutic resistance.

Sub-Nanometer Precision using Bayesian Grouping of Localizations

The paper introduces a Bayesian method to improve localization precision in single-molecule microscopy by grouping localizations from multiple blinking events.

Bayesian Multiple Emitter Fitting using Reversible Jump Markov Chain Monte Carlo

The paper introduces a Bayesian method using Reversible Jump Markov Chain Monte Carlo to fit multiple emitters in dense super-resolution imaging data, providing posterior distributions and model coordinates.

Sequential Super-Resolution Imaging using DNA Strand Displacement

This paper introduces a method for sequential super-resolution imaging using DNA strand displacement to label and remove fluorophores, enabling multiplex imaging of subcellular structures.

Mannan molecular sub-structures control nanoscale glucan exposure in Candida

This study shows that specific N-mannan features in Candida species regulate nanoscale glucan exposure, impacting immune recognition.

Differential Mast Cell Outcomes Are Sensitive to FcϵRI-Syk Binding Kinetics

The paper uses single molecule imaging to study FcϵRI-Syk binding dynamics in mast cells, showing that altered binding kinetics affect signaling outcomes.