K. Gaus

Electrochemically controlled blinking of fluorophores to enable quantitative stochastic optical reconstruction microscopy (STORM) imaging

This paper introduces EC-STORM, an electrochemical method to control fluorophore blinking for STORM imaging, improving molecular counting and imaging quality over conventional photochemical STORM.

Rapid Whole Cell Imaging Reveals A Calcium-APPL1-Dynein Nexus That Regulates Cohort Trafficking of Stimulated EGF Receptor

The paper uses whole-cell imaging to reveal a calcium-APPL1-dynein mechanism that directs EGFR trafficking to the peri-nuclear region.

Precise, high-throughput production of multicellular spheroids with a bespoke 3D bioprinter

This paper develops a 3D bioprinter for high-throughput production of multicellular spheroids and demonstrates its use in drug screening with doxorubicin.

Clustering of CD3ζ is sufficient to initiate T cell receptor signaling

This study shows that clustering of CD3ζ chains alone can initiate TCR signaling, including phosphorylation and calcium flux, in reconstituted cells.

Regulated unbinding of ZAP70 at the T cell receptor by kinetic avidity

The paper shows that ZAP70 unbinding from TCR is regulated by kinetic avidity, allowing both signal fidelity and reversibility in T cell responses.

Geometric regulation of histone state directs melanoma reprogramming

This study identifies a link between geometric features of melanoma aggregates and reprogramming to stem cell-like initiating cells through histone modifications.

tagPAINT: covalent labelling of genetically encoded protein tags for DNA-PAINT imaging

The paper introduces tagPAINT, a covalent labeling method for DNA-PAINT imaging using genetically encoded protein tags, and applies it to study T-cell receptor signaling proteins.

Stoichiometric quantification of spatially dense assemblies with qPAINT

This paper demonstrates that qPAINT measurements remain robust even when target molecules are as close as 3 nm apart, showing no significant cross-reactivity in dense assemblies.

Single molecule localization microscopy with autonomous feedback loops for ultrahigh precision

This paper develops an actively stabilized microscope with feedback loops to improve SMLM precision, enabling nanometer-scale distance measurements in cells.

Conformational states control Lck switching between free and confined diffusion modes in T cells

This study shows that Lck's conformational states determine its diffusive behavior in T cells, with open conformations leading to confined diffusion and closed conformations allowing free diffusion.

Phasor histone FLIM-FRET microscopy quantifies spatiotemporal rearrangement of chromatin architecture during the DNA damage response.

The paper develops a biophysical method to quantify chromatin compaction dynamics during DNA damage response using FLIM-FRET microscopy and identifies roles of ATM and RNF8 in chromatin reorganization.