Journal of Biological ChemistryGeneticsPeer-reviewed journal article
An Alternative, Cas12a-based CRISPR Interference System for Mycobacteria
Abstract
The introduction of CRISPR interference (CRISPRi) has made gene repression in mycobacteria much more efficient, but technical challenges of the prototypical Cas9-based platform, for example in multigene regulation, remain. Here, we introduce an alternative CRSPRi platform that uses the minimal Cas12a enzyme in combination with synthetic CRISPR arrays. This system is simple, tunable, and can regulate multiple genes simultaneously, providing a new tool to probe higher-order genetic interactions in mycobacteria including Mycobacterium tuberculosis (Mtb).
- Citations
- 21
- FWCI
- 0.86
- Field percentile
- top 30%
Reproducibility ledger
Nobody has reported trying to reproduce this paper yet. Did you replicate an experiment or run the code? Your report saves the next lab a week.
Sign in to file a reproducibility report.
Related papers
- Reprogramming the endogenous type III-A CRISPR-Cas system for genome editing, RNA interference and CRISPRi screening in Mycobacterium tuberculosis
- Fluorescence-based CRISPR interference system for controlled genetic repression and live single-cell imaging in mycobacteria
Janïs Laudouze, Vanessa Point, … Pierre Santucci·FEBS LettersPeer-reviewed·Dec 1, 2024
- Development of inducible genetic systems to finely tune efficacy of InhA-targeting anti-tuberculous drugs
Eliana Sepulveda Ramos, Maria Sofia Deschutter, … Pierre Santucci·bioRxiv·Oct 7, 2026
- An isothermal method for sensitive detection of Mycobacterium tuberculosis complexes using CRISPR/Cas12a cis- and trans-cleavage
Haipo Xu, Xiaolong Zhang, … Jingfeng Liu·bioRxiv·Feb 3, 2020
- Mycobacterial cell division arrest and smooth-to-rough envelope transition using CRISPRi-mediated genetic repression systems
Vanessa Point, Wafaa Achache, … Pierre Santucci·FEBS Open BioPeer-reviewed·Nov 30, 2025
- Multiplexed transcriptional repression identifies a network of bactericidal interactions between mycobacterial respiratory complexes