bioRxivBiochemistry
One-Tip enables comprehensive proteome coverage in minimal cells and single zygotes
Z. Ye, P. Sabatier, J. Martin-Gonzalez, A. Eguchi, D. B. Bekker-Jensen, N. Bache, J. V. Olsen
Abstract
We present One-Tip, a lossless proteomics methodology that seamlessly combines swift, one-pot sample preparation with narrow-window data-independent acquisition mass spectrometric analysis. With simplest sample processing, One-Tip reproducibly identifies > 9,000 proteins from [~]1000 cells and [~] 6,000 proteins in a single mouse zygote with a throughput of 40 samples-per-day. This easy-to-use method expands capabilities of proteomics research by enabling greater depth, scalability and throughput covering low to high input samples.
- organism: mouse
- assay: proteomics
- research focus: protein folding & dynamics
- approach: experimental assay
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