Valdemaras Petrosius

This study establishes quality control standards for single-cell proteomics through inter-laboratory benchmarking, identifying software and technical factors affecting data reliability.

This paper introduces CRISPR-PTM and CRISPR-VEIS to quantitatively analyze endogenous phosphosite effects on cellular fitness and signaling, showing Y19 as a novel inhibitory site in the WEE1-CDK1 pathway.

The paper introduces iDIA, a method that enhances sensitivity for quantifying regulatory proteins in single cells, revealing lineage priming and cell-cycle coordination in hematopoietic stem and progenitor cells.

This study evaluates the Orbitrap Astral MS's sensitivity for single-cell proteomics, showing improved proteome coverage and quantitative accuracy in low-input samples.

This study develops a low-input proteomics workflow to analyze rare hematopoietic stem and progenitor cells, identifying 7,000 proteins and a novel marker for long-term hematopoietic stem cells.

The paper evaluates the impact of different trypsin variants on proteomics experiments across multiple sample types and methods, identifying variations in performance and providing handling recommendations.

This study evaluates the Astral mass analyzer's performance in single-cell proteomics using DIA, showing improved proteome depth and quantitative precision for ultra-low input samples.

The paper develops a tailored DIA method for single-cell proteomics to improve quantification from low-input samples and establishes a complete scp-MS workflow.

The paper develops a method combining linear ion traps and FAIMS for low-input proteomics, improving sensitivity and throughput for limited material samples.