Photosynthesis regulation impacts carbon and nitrogen assimilation in the diazotrophic cyanobacterium Anabaena sp. PCC 7120

Abstract

O_LIDiazotrophic cyanobacteria fix both atmospheric carbon (C) and nitrogen (N) into biomass, but the two assimilation pathways are not compatible. Species like Anabaena sp. PCC 7120 physically separates C and N assimilation in different cell types. Even if separated, they are strongly intertwined, as N assimilation relies on the C skeletons and reducing power from photosynthesis, that in turn depends on N rich molecules as pigments and proteins. C_LIO_LIWhereas the two pathways have been extensively studied individually, here we investigate their interaction by analysing photosynthetic properties upon exposure to changes in light, CO2 and N availability, including the contribution of photosynthetic electron fluxes. C_LIO_LIGrowth depended on the availability of both light and CO2, while the N2 fixation activity mainly on the C supply. Upon diazotrophic conditions, the total photosynthetic electron transport activity increased, with a modified contribution of different electron pathways. A mutant strain affected in the vehiculation of fixed N between cell types showed that the modulation of photosynthesis depended on the metabolic connection between assimilation pathways. C_LIO_LIOverall, data showed that the regulation of photosynthetic electron fluxes is a major component of the synergic metabolic relationship between C and N assimilation pathways upon dynamic environmental conditions. C_LI