bioluminescence and chemiluminescence research

ATP-Free Fatty Aldehyde Biosynthesis Enables an Autonomous Lux-Based Bioluminescence System

The paper develops an ATP-free bioluminescence system using DOX to convert fatty acids to fatty aldehydes, enhancing stability under metabolic stress.

A miniature bioluminescent platform for imaging structured RNAs

This paper develops a miniaturized bioluminescent platform for imaging structured RNAs in live cells, achieving a 3-fold reduction in tag size compared to existing methods.

Biosynthetic Olfactory System from Multiplexed Engineered Microbes (BOSMEM)

The paper demonstrates a multiplexed biosensing system using genetically distinct microbial strains in a microfluidic device to monitor multiple chemicals simultaneously with stable signal output.

Biochemical characterisation of fungal bioluminescence enzymes reveals substrate inhibition and secondary turnover of 3-hydroxyhispidin by H3H

Structural mechanism governing radiationless energy transfer in Renilla bioluminescence

This study reports co-crystal structures of a bioluminescent energy-transfer complex in Renilla reniformis, revealing the spatial arrangement of donor-acceptor chromophores and their role in efficient radiationless energy transfer.

Nanoluciferase reporter preserves immunocompetent glioma model fidelity while facilitating longitudinal molecular imaging

This study develops a nanoluciferase reporter system for glioma models that maintains tumor-immune interactions while enabling longitudinal molecular imaging.

Evolved microbial diversity enables combinatoric biosensing in complex environments

The paper develops a framework using evolved microbial diversity and machine learning to enable combinatoric biosensing of multiple analytes in complex environments.

Coupling Luciferase Activation and BRET Enables High-Contrast Red-Window Calcium Imaging

This paper develops two hybrid calcium indicators, HyBRIC and HyBRIC2, that use luciferase activation and BRET for high-contrast red-window calcium imaging with improved brightness and responsiveness.

Development of Shelf-Stable Reagents and Assay Kits for Bioluminescence Applications using the Capillary-Assisted Vitrification Platform Stabilization Technology

This study develops shelf-stable bioluminescence reagents and assay kits using capillary-assisted vitrification technology, demonstrating ambient storage and consistent performance in various assays.

Control of Synaptic Communication through Molecularly Engineered Bioluminescence Light Emission and Sensing

This paper introduces a modular platform called Interluminescence that uses bioluminescent light to control synaptic transmission by activating optogenetic ion channels in postsynaptic neurons.

OxyBLI: A Genetics-Based Approach for In Vivo Real-Time Visualization of Tissue Oxygenation Dynamics

This paper introduces OxyBLI, a noninvasive optical method to monitor tissue oxygenation in real-time, showing how hypoxia and hyperoxia affect oxygen distribution in experimental animals.

Bioluminescent sentinel plants enable autonomous diagnostics of viral infections

This paper develops bioluminescent plants that detect viral infections through spectral changes, using a protease-responsive circuit triggered by viral proteases.

Impact of floral traits on autoluminescence potential in Phalaenopsis orchids

The paper assesses autoluminescence potential in Phalaenopsis orchids by analyzing floral traits and finds that color lightness, organ textures, and epidermal cell types are key predictors.

Differential Kinetics of SARS-CoV-2 Proteases Revealed by a Dual-Color, BRET-based Protease Biosensor, DuProSense

This paper develops a dual-color BRET biosensor to monitor SARS-CoV-2 protease cleavage kinetics in live cells, revealing differential cleavage rates and drug potency.

Next-generation genetically encoded biosensors for spatiotemporal intracellular pH monitoring, mapping, and profiling

This paper develops ORION, a genetically encoded BRET pH sensor with improved performance and broader pH range, and demonstrates its use in identifying acid imprinting states in cancer cells.

A Bioluminescent Activity Dependent Platform, BLADe, for Converting Intracellular Activity to Photoreceptor Activation

The paper introduces BLADe, a platform that converts intracellular activity into photoreceptor activation using bioluminescent sensors and actuators, demonstrating its use in neurons for activity-dependent gene expression and neural dynamics control.

Chemical Stimulation Sustains Bioluminescence of Living Light Materials

This paper develops a chemical stimulation method to sustain bioluminescence in living materials made from Pyrocystis lunula, enabling long-term, responsive light emission.

Bioprospecting Novel Luciferase Genes from Museum Coleoptera

The paper identifies a novel luciferase gene, CRLuc, from a previously unidentified firefly species using DNA extraction and bioinformatic analysis.

Chemogenetic modulation of luciferase emission color for imaging and sensing

This paper introduces LumiFAST, a tunable luciferase that enables customizable red-shifted emission for improved imaging and biosensing in live cells.

Chemiluminescent probes allow for the rapid identification of colibactin-producing bacteria

This study develops chemiluminescent probes to rapidly detect colibactin-producing bacteria in stool samples, showing improved sensitivity and speed over fluorescent alternatives.

High specificity meets genomic flexibility in the Siphamia-Photobacterium symbiosis

The paper investigates the Siphamia-Photobacterium symbiosis, finding that temperate siphonfish hosts exclusively associate with P. mandapamensis, which exhibits genomic and phenotypic flexibility.

Development of a NanoBiT based high throughput screening assay for discovery of NOS1-CAPON interaction inhibitors

The paper develops a NanoBiT-based assay for high-throughput screening of NOS1-CAPON interaction inhibitors, identifying 19 compounds with IC50 values below 30 M.

Advancing Luciferase Activity and Stability beyond Directed Evolution and Rational Design through Expert Guided Deep Learning

This paper develops enhanced NanoLuc luciferase variants using a hybrid approach of deep learning and structure-guided design, improving thermostability and activity at elevated temperatures.

Bright calcium-modulated bioluminescent indicators for activity imaging and red photon-assisted synaptic transmission

The paper introduces improved bioluminescent calcium sensors and opsin variants for non-invasive imaging and synaptic transmission in C. elegans.

A Shot in the Dark: Comparative Morphology of the Bioluminescent Tube Organs in Tubeshoulders (Platytroctidae)

This study presents the first histological analysis of bioluminescent tube organs in tubeshoulders, revealing conserved morphology across species and species-specific anatomical differences.

A coelenterazine-dependent luciferase from the deep-sea coral Anthoptilum murrayi Kölliker, 1880 (Cnidaria: Octocorallia: Pennatulacea)

The paper identifies and characterizes a coelenterazine-dependent luciferase from the deep-sea coral Anthoptilum murrayi, expanding knowledge of luciferase diversity in Cnidaria.

Design of bacterial DNT sensors based on computational models

This study develops a bacterial biosensor for DNT detection using computational models to guide design, achieving improved sensitivity and specificity.

Continuous hypermutation and evolution of luciferase variants

This paper introduces a streamlined platform for luciferase engineering using continuous hypermutation, resulting in improved light output variants without manual library generation.

Stimuli-Expressed Protein Qubits for Quantum Quantification of Heavy Metals in Natural Water

This paper develops a quantum biosensing system using magnetically modulated fluorescent proteins to detect heavy metals in water with high sensitivity and accuracy.

CaBLAM! A high-contrast bioluminescent Ca2+ indicator derived from an engineered Oplophorus gracilirostris luciferase

This paper introduces CaBLAM, a bioluminescent Ca2+ indicator with improved dynamic range and Ca2+ affinity, enabling high-resolution imaging of cellular activity in cultured neurons and in vivo.