Natural Product Screen Identifies Asiatic Acid Targeting Mutant p53

Abstract

Mutations in p53 are common in different cancer types and are reported to protect cancer via different mechanisms. R175H, R248Q, and R273H are the hotspot mutations of p53 and are suggested to increase aerobic glycolysis in cancer cells. Cancer cells rely mostly upon aerobic glycolysis, so it will be interesting to target these three p53 mutants for designing alternative cancer therapy. The class of compounds studied for their potential to target energy metabolism of cancer cells is called mitocans. The current study is an approach to explore if these selected 3 mutants of p53 may act as suitable target(s) for natural mitocans. Hereby, we selected 60 phytocompounds altogether from Andrographis paniculata and Centella asiatica and docked against all three p53 mutants, using Autodock vina. 11 compounds were sorted based on their binding energies and drug-like properties, and toxicity levels prediction showed asiatic acid to be the most significant. As asiatic acid was observed to significantly bind with R248Q only, R248Q-Asiatic acid was identified for molecular dynamics simulation using GROMACS which showed significant interactions. In conclusion, mutant R248Q was observed to be the best target for asiatic acid, though additional in-vitro experiments are important to validate the findings of this study.