iScienceNeurosciencePeer-reviewed journal article
Identity and Nature of Neural Stem Cells in the Adult Human Subventricular Zone
Salma Baig, Javad NadafiD, Redouane Allache, Phuong Uyen Le, Michael LuoiD, Annisa Djedid, Andriniaina Andy Nkili-Meyong, Maryam Safisamghabadi, Alexandre PratiD, Jack Perry AnteliD, Marie‐Christine Guiot, Kevin PetreccaiD
Abstract
The existence of neural stem cells (NSCs) in adult human brain neurogenic regions remains unresolved. To address this, we created a cell atlas of the adult human subventricular zone (SVZ) derived from fresh neurosurgical samples using single-cell transcriptomics. We discovered 2 adult radial glia (RG)-like populations, aRG1 and aRG2. aRG1 shared features with fetal early RG (eRG) and aRG2 were transcriptomically similar to fetal outer RG (oRG). We also captured early neuronal and oligodendrocytic NSC states. We found that the biological programs driven by their transcriptomes support their roles as early-lineage NSCs. Finally, we show that these NSCs have the potential to transition between states and along lineage trajectories. These data reveal that multipotent NSCs reside in the adult human SVZ.
- organism: human
- assay: scRNA-seq
- research focus: cell type annotation
- research focus: clustering
- research focus: trajectory inference
- approach: statistical model
- Citations
- 27
- FWCI
- 4.40
- Field percentile
- top 4%
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