bioRxivCell BiologyPreprint
Sperm Cell Painting: A Mechanism Driven Approach for Drug Discovery in Human Spermatozoa
Z. C. Johnston, F. S. Gruber, R. Myles, S. V. Kane, A. Richardson, D. P. Day, C. Wilson, K. D. Read, I. H. Gilbert, S. Martins da Silva, C. L. R. Barratt, J. R. Swedlow
Abstract
We have adapted the cell painting assay developed by Carpenter and colleagues on cultured U2OS cells to human spermatozoa. In Sperm Cell Painting (SCP) we assemble an image-based quantitative fingerprint of the functional state of sperm. We use this assay to gain insight into the mechanism of action of compounds that modify sperm function and as a platform for contraceptive discovery.
- organism: human
- assay: imaging
- research focus: drug response / drug discovery
- approach: experimental assay
Reproducibility ledger
Nobody has reported trying to reproduce this paper yet. Did you replicate an experiment or run the code? Your report saves the next lab a week.
Sign in to file a reproducibility report.
Related papers
- A phenotypic screening platform utilising human spermatozoa identifies new compounds with contraceptive activity
Franz Sebastian Gruber, Zoe C Johnston, … Paul D. Andrews·eLifePeer-reviewed·Jan 27, 2020
- Human Sperm-Induced Cell-Cell Fusion Requiring JUNO (hSPICER): A paradigm shift to test sperm fertilizing potential
Nicolás Gastón Brukman, Maharan Kabha, … Benjamin Podbilewicz·bioRxiv·May 11, 2026
- A high-throughput flow cytometry assay to evaluate CatSper-mediated Ca2+ influx triggered by high-K+/high-pH stimulation in mouse sperm
Lucila R. Gomez-Olivieri, Liza J. Schiavi‐Ehrenhaus, … Guillermina María Luque·bioRxiv·Jan 5, 2026
- Sperm Toolbox - A selection of small molecules to study human spermatozoa
F. S. Gruber, A. Richardson, … J. R. Swedlow·bioRxiv·May 2, 2023
- Soluble adenylyl cyclase inhibition prevents human sperm functions essential for fertilization
M. Balbach, L. Ghanem, … L. R. Levin·bioRxiv·Apr 27, 2021
- Compounds enhancing human sperm motility identified using a high-throughput phenotypic screening platform
F. S. Gruber, Z. C. Johnston, … C. L. Barratt·Human ReproductionPeer-reviewed·Jan 20, 2022