Stationary phloem proteins and their effects on viruses, aphids, and cyst nematodes in Arabidopsis

The phloem is a specialized tissue that facilitates systemic transport of carbohydrates and signal molecules, making it a common target for viruses, phloem-feeding insects, and cyst nematodes. In Arabidopsis, the stationary phloem-associated SIEVE ELEMENT-LINING CHAPERONE1 (SLI1) and RESTRICTED TEV MOVEMENT (RTM) proteins restrict insect phloem-feeding and potyviruses systemic transport, respectively. However, their broader roles in plant-attacker interactions remain largely unexplored. We investigated the roles of SLI1, RTM1, RTM2, and RTM3 in tobacco etch virus (TEV) infection, as well as in Myzus persicae and Heterodera schachtii infestations using Arabidopsis mutants. Systemic TEV movement was quantified, aphid behaviour and reproduction were assessed, and cyst nematode infection was monitored. SLI1 did not restrict TEV systemic movement, RTM3 reduced M. persicae reproduction without altering feeding behaviour, SLI1, RTM2, and RTM3 supported H. schachtii infection and feeding site expansion, and confocal images indicated a possible role of the proteins in de novo synthesis of sieve tubes around syncytia. These findings reveal that stationary phloem proteins exert dual and target-specific effects, limiting some attackers while inadvertently facilitating others. This highlights the complexity of phloem-based immunity and underscores the need to unravel its underlying mechanisms to develop strategies to reduce multiple pest and pathogen burdens simultaneously. HighlightStationary phloem proteins SLI1, RTM1, RTM2, and RTM3 exert dual and target-specific effects against tobacco etch virus, Myzus persicae, and Heterodera schachtii.