Islet architecture in adult mice is actively maintained by Robo2 expression in beta cells

Abstract

Pancreatic islets of Langerhans have a non-random spatial architecture, which is important to proper glucose homeostasis. Islet architecture forms during embryonic development, in a morphogenesis process partially involving expression of Roundabout (Robo) receptors in {beta} cells, and their ligand, Slit, in the surrounding mesenchyme. Whether islet architecture is set during development and remains passive in adulthood, or whether it requires active maintenance throughout life, has not been determined. To distinguish between these two models, we conditionally deleted Robo2 in {beta} cells of adult mice and observed their islet architecture following a two-month chase. Here, we show that deleting Robo2 in adult {beta} cells causes significant loss of islet architecture without affecting {beta} cell identity, maturation, or stress, indicating that Robo2 plays a role in actively maintaining adult islet architecture. Understanding the factors required to maintain islet architecture, and thus optimize islet function, is important for developing future diabetes therapies.