Entirely synthetic memory inception via dual optogenetic activation of sensory and dopaminergic neurons

Abstract

Associative conditioning is a fundamental learning paradigm that links salient stimuli to appropriate behavior. The vinegar fly is an important memory model that can associate odors with rewarding or punishing reinforcement stimuli via distinct sets of dopaminergic neurons. While much is known about learning, a crucial question remains: is the simple co-activation of sensory and neuromodulatory dopaminergic neurons sufficient to induce memory formation? To address this, we systematically replaced natural stimuli, namely odor-evoked activity, punishment, and reward signals, with optogenetic activation of corresponding neurons, both individually and in combination. In all cases, including a full-substitution paradigm where both olfactory activity and reinforcement dopamine signals were replaced, optogenetic activation successfully implanted synthetic memories. These findings provide a clear answer: simple coincident, rectangular-pulse sensory and neuromodulatory stimulation can instruct associative memory formation. We conclude that associative memory does not have strict requirements for temporal or ensemble activity patterns in olfactory or dopaminergic neurons that are specific to natural stimuli (e.g. odorants, sugar, or pain); rather, simple coincidence is an effective driver of memory inception. These findings open new avenues for manipulating and studying memory formation with all-optical control.