Long-term potentiation in neurogliaform cells modulates excitation-inhibition balance in the temporoammonic pathway

Abstract

Apical dendrites of pyramidal neurons integrate information from higher-order cortex and thalamus, and gate signaling and plasticity at proximal synapses. In the hippocampus, neurogliaform cells and other interneurons located within stratum lacunosum-moleculare mediate powerful inhibition of CA1 pyramidal neuron distal dendrites. Is the recruitment of such inhibition itself subject to use-dependent plasticity, and if so, what induction rules apply? Here we show that interneurons in mouse stratum lacunosum-moleculare exhibit Hebbian NMDA receptor-dependent long-term potentiation (LTP). Such plasticity can be induced by selective optogenetic stimulation of afferent fibers in the temporoammonic pathway from the entorhinal cortex, but not by equivalent stimulation of afferents from the thalamic nucleus reuniens. We further show that theta-burst patterns of afferent firing induces LTP in neurogliaform interneurons identified using neuron-derived neurotrophic factor (Ndnf)-Cre mice. Theta-burst activity of entorhinal cortex afferents led to an increase in disynaptic feed-forward inhibition, but not monosynaptic excitation, of CA1 pyramidal neurons. Activity-dependent synaptic plasticity of neurogliaform cells in stratum lacunosum-moleculare thus alters the excitation-inhibition balance at entorhinal cortex inputs to the apical dendrites of pyramidal neurons, implying a dynamic role for these interneurons in gating CA1 dendritic computations. Significance statementElectrogenic phenomena in distal dendrites of principal neurons in the hippocampus have a major role in gating synaptic plasticity at afferent synapses on proximal dendrites. Apical dendrites also receive powerful feed-forward inhibition mediated in large part by neurogliaform neurons. Here we show that theta-burst activity in afferents from the entorhinal cortex induces Hebbian long-term potentiation at excitatory synapses recruiting these GABAergic cells. Such LTP increases disynaptic inhibition of principal neurons, thus shifting the excitation-inhibition balance in the temporoammonic pathway in favor of inhibition, with implications for computations and learning rules in proximal dendrites.