PLoS ONEGenomicsPeer-reviewed journal article
Single Molecule Sequencing Of M13 Virus Genome Without Amplification
Luyang ZhaoiD, Liwei DengiD, Gailing LiiD, Huan Jin, Jinsen CaiiD, Huan ShangiD, Yan LiiD, Haomin Wu, Weibin XuiD, Lidong ZengiD, Renli ZhangiD, Huan ZhaoiD, Ping Chun WuiD, Zhiliang Zhou, Jiao ZhengiD, Pierre Ezanno, Andrew X. YangiD, Qin Yan, Michael W. DeemiD, Jiankui HeiD
- Citations
- 16
- FWCI
- 0.89
- Field percentile
- top 28%
Abstract
Third generation sequencing is a direct measurement of DNA/RNA sequences at the single molecule level without amplification. In this study, we report sequencing of the genome of the M13 virus by a new single molecule sequencing platform. Our platform detects single molecule fluorescence by the total internal reflection microscope technique, with sequencing-by-synthesis chemistry. We sequenced the genome of M13 to a depth of 316x and 100% coverage. The consensus sequence accuracy is 100%. We demonstrated that single molecule sequencing has no significant GC bias.
Reproducibility ledger
Nobody has reported trying to reproduce this paper yet. Did you replicate an experiment or run the code? Your report saves the next lab a week.
Sign in to file a reproducibility report.
Related papers
- Resequencing the Escherichia coli genome by GenoCare single molecule sequencing platform
Luyang Zhao, Liwei Deng, … Jiankui He·bioRxiv·Jul 13, 2017
- Rapid Sequencing of Multiple RNA Viruses in their Native Form
Thidathip Wongsurawat, Piroon Jenjaroenpun, … Intawat Nookaew·Frontiers in MicrobiologyPeer-reviewed·Feb 25, 2019
- Two Color Single Molecule Sequencing on GenoCare 1600 Platform to Facilitate Clinical Applications
- CODEC enables 'single duplex' sequencing
J. H. Bae, R. Liu, … V. A. Adalsteinsson·bioRxiv·Jun 11, 2021
- Cost-efficient whole genome-sequencing using novel mostly natural sequencing-by-synthesis chemistry and open fluidics platform
Gilad Almogy, Mark Pratt, … Doron Lipson·bioRxiv·May 29, 2022
- Error correction and assembly complexity of single molecule sequencing reads.
Hayan Lee, James Gurtowski, … Michael Schatz·bioRxiv·Jun 18, 2014