Complete Genome Sequence of Six Pseudomonas aeruginosa Bacteriophages aim for research and treatments

Abstract

IntroductionPseudomonas aeruginosa is a major opportunistic pathogen associated with healthcare-associated infections. The rise of antibiotic-resistant strains necessitates alternative treatment strategies, with bacteriophage therapy being a promising approach. MethodsSix bacteriophages were isolated from sewage samples. Phage isolation involved centrifugation, filtration, and plaque assays. The morphology of each was examined using Transmission Electron Microscopy (TEM). Genomic DNA was sequenced analyzed and compared. Phages lytic activity was assessed using growth curve analysis. ResultsThe six phages displayed distinct genomic and morphological characteristics, in three genomic clusters. No known virulence or antibiotic resistance genes were detected, indicating their safety for therapeutic use. TEM analysis revealed diverse morphologies, with some phages belonging to the Siphoviridae family and others to the Myoviridae family. Lysogenic phages demonstrated less effective lytic activity. ConclusionSome of these phages are promising candidates for the research of phage therapy efficacy, and the lytic phages can be used against P. aeruginosa infections.