Two-photon microscopy at >500 volumes/second

Y.-H. Tsai; C.-W. Liu; W.-K. Lin; C.-S. Wang; C.-H. Chiang; V. R. Singh; P. T. C. So; C.-F. Chou; S.-W. Chu

doi:10.1101/2020.10.21.349712

BiophysicsbioRxivOct 21, 2020

Preprint

Two-photon microscopy at >500 volumes/second

Y.-H. Tsai, C.-W. Liu, W.-K. Lin, C.-S. Wang, C.-H. Chiang, V. R. Singh, P. T. C. So, C.-F. Chou, S.-W. Chu

Abstract

We demonstrate a multi-focal multi-photon volumetric microscopy via combination of 32-beam parallel lateral-scanning, a 70-kHz axial-scanning acoustic lens, and a 32-channel photodetector, enabling unprecedented data rate (2-10 GHz) and >500-volumes/second imaging speed over ~200x200x200-m3.

PDF DOI