Kaspar Podgorski

Measuring neuronal input-output operations in vivo with random access projection microscopy

This paper introduces SLAP2, a high-speed microscope for measuring submicron synapses and dendritic voltage in behaving mice, revealing nonlinear input-output amplification in neurons.

Dynamo: An open-source Python application for quantifying neuronal structural plasticity over time

Dynamo quantifies neuronal structural plasticity over time by streamlining morphometric analysis of dynamic structural changes in neurons.

GIAnT: a Glutamate Imaging Analysis Toolbox

GIAnT is a toolbox for analyzing glutamate imaging data that corrects motion, identifies active synapses, and improves signal quality.

A sensitive orange fluorescent calcium ion indicator for imaging neural activity

The paper introduces OCaMP, an orange fluorescent calcium indicator that improves sensitivity and performance compared to existing green and red indicators for neural imaging.

Ultrasensitive voltage imaging reveals distinct electrical microdomains in neurons

The paper develops a genetically encoded voltage indicator to measure subthreshold voltage dynamics in neurons, revealing electrical diversity and local computation.

Glutamate indicators with increased sensitivity and tailored deactivation rates

The paper develops two variants of a glutamate indicator with enhanced sensitivity and tailored deactivation rates for studying synaptic transmission in vivo.

Far-red fluorescent genetically encoded calcium ion indicators

This paper introduces two new far-red fluorescent GECIs, FR-GECO1a and FR-GECO1c, characterized for their Ca2+ sensitivity and performance in neuronal imaging.

A novel rhodopsin-based voltage indicator for simultaneous two-photon optical recording with GCaMP in vivo

The paper introduces a novel rhodopsin-based voltage indicator, 2Photron, enabling simultaneous two-photon recording of spiking and subthreshold voltage with GCaMP in awake mice.

A blue-shifted genetically encoded Ca2+ indicator with enhanced two-photon absorption

The paper develops T-GECO1, a blue-shifted genetically encoded Ca2+ indicator with enhanced two-photon brightness for improved neuronal activity imaging.

Enhancer-AAVs allow genetic access to oligodendrocytes and diverse populations of astrocytes across species

The paper develops AAV-based genetic tools to specifically target astrocytes and oligodendrocytes across species, revealing functional roles in reward-dependent acetylcholine responses.

Glutamate indicators with improved activation kinetics and localization for imaging synaptic transmission

This paper develops improved glutamate indicators with faster activation kinetics and better synaptic localization for imaging synaptic transmission in neurons.

Improved genetically encoded fluorescent biosensors for monitoring of intra- and extracellular L-lactate

This paper develops improved fluorescent biosensors for monitoring extracellular and intracellular L-lactate in mammalian cells, showing enhanced sensitivity and response kinetics.

GESIAP: A Versatile Genetically Encoded Sensor-based Image Analysis Program

GESIAP is a MATLAB-based image analysis program that processes genetically encoded sensor data to analyze neuromodulator transmission properties across cell types and species.

Activity-driven synaptic translocation of LGI1 controls excitatory neurotransmission

This study shows that neuronal activity drives the translocation of LGI1 and ADAM23 to the synaptic cleft, adjusting excitatory neurotransmission based on activity history.

A genetically encoded fluorescent biosensor for extracellular L-lactate

This paper develops a genetically encoded fluorescent biosensor for extracellular L-lactate and demonstrates its use in imaging in cultured mammalian cells and brain tissue.

VolPy: automated and scalable analysis pipelines for voltage imaging datasets

VolPy provides an automated, scalable pipeline for analyzing voltage imaging datasets, demonstrating strong performance in neuron localization and spike extraction.

Comprehensive imaging of synaptic activity reveals dendritic growth rules that cluster inputs

The paper shows sensory input directs dendritic growth and synapse clustering in developing neurons through activity-dependent pruning and new synaptogenesis.

A general approach to engineer positive-going eFRET voltage indicators

The paper describes engineering eFRET voltage indicators with positive-going fluorescence responses by modifying microbial rhodopsins.

A bright and high-performance genetically encoded Ca2+ indicator based on mNeonGreen fluorescent protein

The paper develops a new genetically encoded Ca2+ indicator, mNG-GECO1, based on mNeonGreen, which is brighter than GCaMP6s and performs comparably in various imaging applications.

jYCaMP: An optimized calcium indicator for two-photon imaging at fiber laser wavelengths

The paper introduces jYCaMP1, a calcium indicator optimized for two-photon imaging at wavelengths above 1000 nm, showing improved performance in mice and flies.

A fast genetically encoded fluorescent sensor for faithful in vivo acetylcholine detection in mice, fish, worms and flies

iAChSnFR, a fluorescent indicator built from a bacterial periplasmic binding protein, reports acetylcholine with large, fast signals in mouse, fish, fly and worm preparations.

Two-photon Imaging with Silicon Photomultipliers

This study compares SiPMs and GaAsP PMT for two-photon imaging, finding SiPMs match PMT signal-to-noise ratio at typical calcium imaging rates and outperform at higher rates.

Bright and photostable chemigenetic indicators for extended in vivo voltage imaging

The paper introduces a new genetically encoded voltage indicator, Voltron, which improves brightness and photostability for extended in vivo voltage imaging in mice, zebrafish, and fruit flies.

Kilohertz frame-rate two-photon tomography

This paper introduces a two-photon imaging technique that achieves high-speed, high-resolution imaging of neural activity in mice at over 1 kHz frame rates.

A genetically encoded fluorescent sensor for in vivo imaging of GABA

This paper develops a genetically encoded fluorescent sensor, iGABASnFR, to monitor GABA in vivo and demonstrates its use in tracking GABA dynamics in various neural contexts.

Stability, affinity and chromatic variants of the glutamate sensor iGluSnFR

The paper introduces variants of the glutamate sensor iGluSnFR with improved brightness, broader concentration detection, and different emission colors for enhanced in vivo imaging.

Multiplicative Updates for Optimization Problems with Dynamics

The paper develops multiplicative update rules for optimizing convex objectives with nonnegativity constraints and applies them to signal processing tasks using neuronal calcium imaging and electrophysiology data.