Tissue recoil in the early Drosophila embryo is a passive not active process

Abstract

Understanding tissue morphogenesis is impossible without knowing the mechanical properties of the tissue being shaped. Although techniques for measuring tissue material properties are continually being developed, methods for determining how individual proteins contribute to mechanical properties are very limited. Here, we developed two complementary techniques for the acute inactivation of sqh (the Drosophila myosin regulatory light chain), one based on the recently introduced AID2 system, and the other based on a novel method for conditional protein aggregation. Combining these techniques with rheological measurements, we show that passive material properties of the cellularization-stage Drosophila embryo are essentially unaffected by myosin activity. The significance of this study is two-fold. We introduce a system for the nearly instantaneous inactivation of proteins in a variety of systems. Additionally, we demonstrate a method to distinguish between active and passive contributions to effective tissue elasticity. SummaryTechniques to examine the contribution of specific proteins to tissue mechanical properties are extremely limited. Here, Goldner et al. develop two complementary techniques for rapid protein depletion combined with mechanical measurements, and show that myosin activity is dispensable for tissue elasticity.