Virus-based gene therapy research

Global connectivity and function of descending spinal input revealed by 3D microscopy and retrograde transduction

This study uses retrograde viral vectors and 3D microscopy to map supraspinal connectivity in the spinal cord and demonstrate their therapeutic potential.

Lentiviral co-packaging mitigates the effects of intermolecular recombination and multiple integrations in pooled genetic screens

Diluting lentiviral perturbation libraries with a carrier plasmid during packaging prevents intermolecular recombination and multiple integrations in pooled screens, although viral titer drops by 100-fold.

Efficient lentiviral transduction of different human and mouse cells

This study describes optimized techniques for efficient lentiviral transduction of mammalian and human cells, achieving high transgene expression levels.

Widespread and targeted gene expression by systemic AAV vectors: Production, purification, and administration

This paper describes a protocol for producing and administering AAV-PHP vectors to label and manipulate cells in the mouse nervous system and organs, with guidelines for experimental design.

Zika virus infection induces synthesis of Digoxin in glioblastoma cells

This study investigates ZIKV infection effects on glioblastoma cells, identifying Digoxin as a biomarker associated with cytopathic effects and suggesting potential oncolytic applications.