Avtar K Singh

This study uses single-cell image-based CRISPR screens to identify host factors regulating Ebola virus infection, revealing new therapeutic targets like UQCRB and STRAP.

The paper presents a genome-wide perturbation atlas using CRISPR-Cas9 knockouts to link genes to cellular phenotypes, identifying TMEM251/LYSET as a Golgi-resident protein involved in lysosomal enzyme trafficking.

PerturbView enables highly multiplexed optical pooled screens in primary cells and tissues by improving barcode amplification, revealing regulators of NFκB signaling and enabling spatial transcriptomics integration.

The paper introduces an optical method to link genetic perturbations with phenotypic outcomes in a pooled setting, identifying novel regulators of immune responses.

Diluting lentiviral perturbation libraries with a carrier plasmid during packaging prevents intermolecular recombination and multiple integrations in pooled screens, although viral titer drops by 100-fold.