Discover, rate, and discuss biology preprints.

This paper describes a biomimetic system for reconstituting actin assembly on functionalized bead surfaces to study regulation mechanisms.

The paper characterizes 29 nanobodies targeting BasC using biochemical assays, structural data, and AlphaFold3, revealing a bias toward intracellular binding and validating epitope clusters.

This study identifies a reversible conformational switch in CdaR's YbbR domains that senses extracellular pH and regulates c-di-AMP signaling in Gram-positive bacteria.

This paper develops a HER2-targeted degrader-antibody conjugate that selectively degrades cyclin K in HER2-positive breast cancer cells.

This study identifies critical experimental parameters affecting Gaussia luciferase activity measurements and proposes a framework for optimizing assay conditions to improve reproducibility.

This study evaluates P2-based plasma enrichment for proteome coverage and biological consistency across preanalytical variables and cancer cohorts.

The paper reassesses whether SGLT2 inhibitors directly activate PANK1 and finds they do not, challenging the proposed cardioprotective mechanism.

The paper characterizes glucosinolate chemodiversity in Cardamine hirsuta accessions and wild-collected material, identifying genetic sources of variation in glucosinolate composition across organs and accessions.

This study uses proteomics to identify molecular changes during AKI to CKD progression in mice, finding shared and distinct pathways and potential biomarkers.

This paper introduces xGL-APMS to profile endogenous galectin-3 interactions at the cell surface, identifying 125 interactors on Jurkat cells and 69 on mouse thymocytes.

This study determines the structure of full-length human hephaestin using cryo-EM, revealing its homodimeric organization and substrate-binding site.

This study identifies SUMOylation and non-proteolytic ubiquitination as mechanisms regulating IRF2BP2 nuclear localization and stability through a SUMO1-NLS-importin-β pathway.

This study identifies molecular mechanisms underlying β1-adrenoceptor selectivity of antagonists through cryo-EM structures, kinetic analysis, and MD simulations.

This study compares proteomic responses to lead exposure in house sparrows from two Australian towns, finding divergent molecular strategies in proteostasis and haem biosynthesis.

This study shows that long-term estrogen deprivation protects against acetaminophen-induced liver injury through lipid remodeling and sustained NRF2 activation.

This study identifies a proteomic signature of aging in female skeletal muscle linked to physical function decline using proteomic profiling and clinical measurements.

This study shows that SAM supply regulates DNA methylation turnover in synovial sarcoma, with SAM depletion causing methylation redistribution rather than global hypomethylation.

The paper reanalyzes proteomic data to identify detection limits for acquired lysines in cancer, finding low recovery rates and high false discovery proportions due to technical challenges.

This paper designs de novo ligases that achieve site-specific ubiquitylation of a target protein with high selectivity and efficiency.

This study identifies HRG as a plasma biomarker of Ni-Cr exposure and reveals nuclear toxicity linked to DNA damage and epigenetic disruption in house sparrows.