Discover, rate, and discuss biology papers.

The paper introduces robustness measures to evaluate cell-type classification reliability in single-cell RNA-seq datasets of kidney organoids and finds high robustness in most cell types except immature ones.

The paper presents the first T2T genome assembly of Eucalyptus recurva, revealing genomic features and conservation insights.

A back-splicing circRNA reporter yields linear trans-spliced artifacts; in orthogonal assays three reported cellular IRESes are inactive, and Iso-Seq 5' ends agree with nAnT-iCAGE start sites.

The paper constructs a graph-based pangenome of Venturia inaequalis, identifies structural variants linked to population differentiation, and reveals gene repertoire conservation and virulence-associated diversity.

This study identifies three functional programs impacted by autism genes, contextualized across neuronal subclasses, developmental timing, and cortical regions.

The paper evaluates methods for detecting and characterizing inversions in Timema stick insects, showing differences in their ability to identify and describe inversion structures.

The paper identifies an insertion-deletion variant in a super-enhancer regulating LINC00636 and CD47 in breast cancer, showing its impact on chromatin accessibility and tumor-promoting features.

The paper identifies an almost-complete Rickettsia africae chromosome in the genome of its arthropod vector, A. variegatum, demonstrating lateral gene transfer from the pathogen to the host.

This study uses pangenomics and machine learning to identify genetic markers associated with carotenoid accumulation in sorghum, revealing structural variation and multi-locus genetic architecture.

The paper introduces taxutils, a tool for managing NCBI taxonomy and accession mappings, and demonstrates its use in assessing assembly quality through taxonomic mapping analysis.

The paper identifies convergent gene losses and expression changes associated with blood-feeding loss in non-blood-feeding mosquitoes.

The paper develops crop-specific Nanopore basecalling models for Rosaceae species to improve sequencing quality and enables community use of these models.

The paper develops a GT-seq panel to identify species in the Coregonus artedi complex and demonstrates its cross-amplification in related taxa.

PeakATail identifies poly(A)-sites in single-cell RNA-seq with precision, controlling error rates and validating results against curated data.

The paper uses whole genome sequencing to analyze Vibrio cholerae O1 isolates from 2023-2024 European cholera cases, confirming their lineage and identifying drug-resistant clones.

CMAC estimates absolute transcriptional age of cardiomyocytes using deep learning, achieving high accuracy across multiple datasets and platforms.

The paper presents the first high-quality genome assembly of a strepsipteran species and compares it with two others, finding a conserved genomic blueprint for obligate endoparasitism.

The paper identifies two new bat coronavirus subgenera and potential species from Neotropical bats using metatranscriptomic sequencing and phylogenetic analysis.

CHART-seq enables ultra-fast, scalable full-length single-cell RNA sequencing with improved gene detection and isoform annotation compared to existing methods.

The paper shows that isoleucine deficiency in human hemoglobin mirrors mosquito proteomes and limits malaria parasite growth.