Small-molecule binding to an intrinsically disordered protein revealed by experimental NMR 19F transverse spin-relaxation
G. T. Heller, V. K. Shukla, A. M. Figueiredo, D. F. Hansen
This study shows that 19F transverse relaxation rates from NMR can detect small-molecule binding to intrinsically disordered proteins, demonstrating 5-fluoroindole binds to HCV NS5A with a Kd of 260 ± 110 M.
bioRxiv · May 3, 2023Biophysics