A. N. Edwards

The pH-responsive SmrR-SmrT system modulates C. difficile antimicrobial resistance, spore formation, and toxin production

This study identifies SmrR and SmrT as pH-responsive genes in C. difficile that regulate sporulation, toxin production, and antimicrobial resistance.

The predicted acetoin dehydrogenase pathway represses sporulation of Clostridioides difficile

This study shows that acetoin and pH regulate sporulation in C. difficile through the aco pathway, with acetoin metabolism not supporting growth as a stationary phase energy source.

Glycine fermentation by C. difficile promotes virulence, spore formation, and is induced by host cathelicidin

This study shows that glycine fermentation by C. difficile enhances virulence, spore formation, and is triggered by host cathelicidin.

The RgaS-RgaR two-component system promotes Clostridioides difficile sporulation through a small RNA and the Agr1 system

This paper identifies the RgaS-RgaR two-component system as a regulator of sporulation in C. difficile through two pathways involving a small RNA and a quorum-sensing peptide.

A Conserved Switch Controls Virulence, Sporulation, and Motility in C. difficile

This study identifies a conserved molecular switch involving Spo0E that regulates sporulation, motility, and virulence in C. difficile.

Identification of functional Spo0A residues critical for sporulation in Clostridioides difficile

This study identifies functional Spo0A residues critical for sporulation in C. difficile by analyzing mutations in conserved regions and their effects on spore formation.

c-di-GMP inhibits early sporulation in Clostridioides difficile

This study shows that c-di-GMP inhibits early sporulation in C. difficile by reducing sporulation frequency and gene transcription, suggesting a regulatory role in spore formation.

Three orphan histidine kinases inhibit Clostridioides difficile sporulation

This study identifies three phosphotransfer proteins that repress Clostridioides difficile sporulation by regulating Spo0A phosphorylation.

RstA Regulation of Clostridioides difficile Toxin Production and Sporulation in Phenotypically Diverse Strains

This paper investigates RstA's role in regulating toxin production and sporulation in diverse C. difficile strains, showing strain-dependent effects and sequence-specific promoter interactions.

Characterization of a β-lactamase that contributes to intrinsic β-lactam resistance in Clostridioides difficile

This study identifies and characterizes a β-lactamase, BlaD, in C. difficile that contributes to β-lactam resistance, showing its regulation by BlaRI and the role of a co-transcribed lipoprotein, BlaX.

RstA is a Major Regulator of Clostridioides difficile Toxin Production and Motility

This paper identifies RstA as a transcriptional repressor that inhibits toxin gene expression and positive regulators in C. difficile, revealing its role in controlling toxin production through direct DNA binding and autoregulation.

The C. difficile clnRAB operon initiates adaptations to the host environment in response to LL-37

This paper identifies the C. difficile clnRAB operon as a regulatory system that responds to LL-37, enabling adaptation to the host intestinal environment.