Alexander Pisera

A System to Explore the Adaptive Dynamics of Multicopy Plasmids: The Role of Copy Number and Mutation Rate in Evolutionary Outcomes

This study investigates how plasmid copy number and mutation rate affect evolutionary outcomes using the OrthoRep system, finding that low copy number accelerates beneficial allele enrichment while high copy number increases robustness and hitchhiking of deleterious mutations.

Ultra-Efficient Integration of Gene Libraries onto Yeast Cytosolic Plasmids

The paper introduces a method to efficiently integrate gene libraries onto yeast cytosolic plasmids using integrases, enhancing the versatility of OrthoRep for protein engineering.

Continuous evolution of gene libraries towards arbitrary functions reveals the versatility of biomolecular evolution in vivo

This study shows that novel gene functions can emerge rapidly in vivo through continuous evolution under various selection pressures.

Continuous evolution of user-defined genes at 1-million-times the genomic mutation rate

This paper introduces a method to rapidly evolve genes in the lab, revealing new insights into enzyme adaptation that machine learning models cannot predict.