Discovery of a single-subunit oligosaccharyltransferase that enables glycosylation of full-length IgG antibodies in Escherichia coli
Belen Sotomayor, Thomas Connor Donahue, Sai Pooja Mahajan, May N. Taw, Sophia W. Hulbert, Erik J. Bidstrup, D. Natasha Owitipana, Alexandra Pang, Yang Xu, Souvik Ghosal, Christopher A. Alabi, Parastoo Azadi, Jeffrey J. Gray, Michael C. Jewett, Lai‐Xi Wang, Matthew P. DeLisa
The paper identifies a single-subunit oligosaccharyltransferase from Desulfovibrio marinus that enables glycosylation of full-length IgG antibodies in E. coli.
bioRxiv · Aug 12, 2024 · PreprintSynthetic Biology