Exploiting the inherent promiscuity of the acyl transferase of the stambomycin polyketide synthase for the mutasynthesis of analogues
Ли Су, Souaibou Yaouba, Laurence Hôtel, Christophe Jacob, Peter Grün, Yan‐Ni Shi, Alicia Chateau, Sophie Pinel, Helge B. Bode, Bertrand Aigle, Kira J. Weissman
This paper uses mutasynthesis to generate stambomycin derivatives by engineering a bacterial strain and leveraging a promiscuous acyl transferase domain, resulting in new analogues with similar or reduced bioactivity.
Chemical Science · Jan 1, 2025Peer-reviewedSynthetic Biology