Synthetic BiologyChemical SciencePeer-reviewed
Exploiting the inherent promiscuity of the acyl transferase of the stambomycin polyketide synthase for the mutasynthesis of analogues
Ли Су, Souaibou Yaouba, Laurence Hôtel…·Kira J. Weissman
This paper uses mutasynthesis to generate stambomycin derivatives by engineering a bacterial strain and leveraging a promiscuous acyl transferase domain, resulting in new analogues with similar or reduced bioactivity.