A cell-free biosynthesis platform for modular construction of protein glycosylation pathways
Weston Kightlinger, Katherine E. Duncker, Ashvita Ramesh, Ariel Helms Thames, Aravind Natarajan, Jessica C. Stark, Allen Yang, Lin Liang, Milan Mrksich, Matthew P. DeLisa, Michael C. Jewett
The paper introduces a cell-free platform for assembling protein glycosylation pathways and demonstrates its use in creating vaccine proteins and antibody constant regions with specific glycan motifs.
Nature Communications · Nov 27, 2019Peer-reviewedSynthetic Biology