Weston Kightlinger

Scalable cell-free production of active T7 RNA polymerase

This paper demonstrates scalable cell-free production of active T7 RNA polymerase with high purity and activity, suitable for decentralized biotherapeutic manufacturing.

Point-of-care peptide hormone production enabled by cell-free protein synthesis

The paper develops a platform for point-of-care synthesis of peptide hormones using cell-free protein synthesis and affinity purification, enabling local production of therapeutics in resource-limited settings.

A high-throughput, automated, cell-free expression and screening platform for antibody discovery

This paper develops an automated cell-free platform for rapid antibody discovery and screening, demonstrating its effectiveness with SARS-CoV-2 neutralizing antibodies.

A cell-free biosynthesis platform for modular construction of protein glycosylation pathways

The paper introduces a cell-free platform for assembling protein glycosylation pathways and demonstrates its use in creating vaccine proteins and antibody constant regions with specific glycan motifs.

A cell-free platform for rapid synthesis and testing of active oligosaccharyltransferases

This paper presents a cell-free platform for synthesizing active oligosaccharyltransferases (OSTs) and demonstrates its ability to produce and test multiple OSTs for glycosylation reactions.