Harnessing Diversity Generating Retroelements for in vivo targeted hyper-mutagenesis
Raphaël Laurenceau, Paul Rochette, Elena Lopez-Rodriguez, Catherine Fan, Amandine Maire, Paul Vittot, Karol Melissa Cerdas-Mejías, Auguste Bouvier, Thea Chrysostomou, David Bikard
This paper introduces DGRec, a method using DGRs to enable targeted hyper-mutagenesis in E. coli, creating diverse variants that allow evasion of phage infection.
bioRxiv · Mar 24, 2025 · PreprintSynthetic Biology