B. Belardi

Extracellular Electron Transfer Enables Cellular Control of Cu(I)-catalyzed Alkyne-Azide Cycloaddition

This study uses extracellular electron transfer from Shewanella oneidensis to control Cu(I)-catalyzed Alkyne-Azide Cycloaddition reactions and enables bioorthogonal labeling on mammalian cells.

Biased localization of actin binding proteins by actin filament conformation

The paper shows that actin-binding proteins preferentially localize to different actin structures based on filament conformation, using live cell imaging and in vitro binding measurements.

Molecular height measurement by cell surface optical profilometry (CSOP)

The paper introduces CSOP, a method to measure molecular heights on cell surfaces with nanometer resolution, and finds that protein height is smaller than contour length and depends on surface and solution conditions.

A weak link with actin organizes tight junctions to control epithelial permeability

This study identifies a weak ZO-1-actin interaction that controls epithelial permeability through kinetic trapping, enabling tunable permeability in engineered monolayers.

Steric Regulation of Tandem Calponin Homology Domain Actin-Binding Affinity

The paper investigates how structural modifications affect the actin-binding affinity of tandem CH1-CH2 domains, finding that steric changes and interface mutations influence binding properties and sub-cellular localization.

Claudin-4 Reconstituted in Unilamellar Vesicles is Sufficient to Form Tight Interfaces that Partition Membrane Proteins

This study shows that claudin-4 reconstituted in vesicles forms tight interfaces that partition membrane proteins, supporting its role as a molecular fence in epithelial polarization.