Click-Based Determination of Accumulation of Molecules in Escherichia coli
G. M. Ongwae, Z. Liu, S. Feng, M. D. Chordia, R. Dash, Y. He, M. Sharifian Gh., B. E. Dalesandro, T. Guo, K. B. Sharpless, J. Dong, M. S. Siegrist, W. Im, M. Pires
The paper develops CHAMP, a method to measure cytosolic accumulation of azide-tagged molecules in Gram-negative bacteria, enabling high-throughput screening of over 1,000 compounds.
bioRxiv · Jun 20, 2023Microbiology