Author

M. S. Siegrist

Listed by name: these papers carry no ORCID for this author, so different people with the same name may be mixed together.

Biology Disciplines

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Microbiology· 10 Biochemistry· 1

Signature Assays, Organisms & Methods

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Approach:experimental assay· 10 Research Focus:cell signaling & trafficking· 6 Organism:bacteria (other)· 5 Research Focus:cell type annotation· 5 Assay:proteomics· 5 Research Focus:development & morphogenesis· 3 Organism:mouse· 3 Research Focus:drug response / drug discovery· 2

Frequent Co-Authors

Y. S. Morita· 5 shared T. Kado· 4 shared A. Garcia-Heredia· 3 shared B. E. Dalesandro· 3 shared E. S. Melzer· 3 shared M. D. Chordia· 3 shared A. A. Pohane· 2 shared A. J. Apostolos· 2 shared

Activity by Year

2018-2023 · peak 4/yr

Co-Author Collaboration Network

10 top collaborators · 20 mutual co-author links

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5 shared papersMicrobiology

Y. S. Morita

Mutual co-authors in cluster

Author profile →

11 papers

Recent·Most cited

Click-Based Determination of Accumulation of Molecules in Escherichia coli

G. M. Ongwae, Z. Liu, S. Feng, M. D. Chordia, R. Dash, Y. He, M. Sharifian Gh., B. E. Dalesandro, T. Guo, K. B. Sharpless, J. Dong, M. S. Siegrist, W. Im, M. Pires

The paper develops CHAMP, a method to measure cytosolic accumulation of azide-tagged molecules in Gram-negative bacteria, enabling high-throughput screening of over 1,000 compounds.

bioRxiv · Jun 20, 2023Microbiology

Permeation Across the Mycomembrane in Live Mycobacteria

Z. Liu, I. Lepori, B. E. Dalesandro, M. D. Chordia, J. Dong, M. S. Siegrist, M. Pires

The paper develops a novel assay to measure small molecule permeation across the mycomembrane of mycobacteria, revealing variability in permeability profiles between species.

bioRxiv · Oct 12, 2022 · PreprintMicrobiology

Genetic and lipidomic identification of tuberculostearic acid as a controller of mycobacterial membrane compartmentalization

M. Prithviraj, T. Kado, J. A. Mayfield, D. C. Young, A. D. Huang, D. Motooka, S. Nakamura, M. S. Siegrist, D. B. Moody, Y. S. Morita

The paper identifies tuberculostearic acid (TBSA) as a key lipid controlled by the gene cfa, which regulates membrane compartmentalization in Mycobacterium smegmatis.

bioRxiv · Aug 17, 2022 · PreprintMicrobiology

Inositol acylation of phosphatidylinositol mannosides: a rapid mass response to membrane fluidization in mycobacteria

P. P. Nguyen, T. Kado, M. Prithviraj, M. S. Siegrist, Y. S. Morita

This study shows that mycobacteria rapidly modify phosphatidylinositol mannosides (PIMs) by adding an inositol acyl chain in response to membrane fluidization, enhancing resistance to membrane stress.

bioRxiv · Jul 23, 2022Biochemistry

Compartment-Specific Measurement of Small Molecule Accumulation into Diderm Bacteria

G. M. Ongwae, I. Lepori, M. D. Chordia, B. E. Dalesandro, A. J. Apostolos, M. S. Siegrist, M. M. Pires

The paper develops BaCAPA, a method to measure small molecule accumulation in specific bacterial compartments, showing its utility for both model and pathogenic bacteria.

bioRxiv · May 5, 2022 · PreprintMicrobiology

Metabolic Processing of Selenium-based Bioisostere of meso-diaminopimelic Acid in Live Bacteria

A. J. Apostolos, T. M. Koyasseril-Yehiya, C. Santamaria, J. R. A. Silva, J. Lameira, M. C. Alves, M. S. Siegrist, M. M. Pires

The paper synthesizes and demonstrates the metabolic processing of a selenium-based bioisostere of m-DAP in live bacteria, showing its incorporation into the peptidoglycan layer.

bioRxiv · Dec 18, 2021Microbiology

Cell wall damage reveals spatial flexibility in peptidoglycan synthesis and a non-redundant role for RodA in mycobacteria

E. S. Melzer, T. Kado, A. Garcia-Heredia, K. R. Gupta, X. Meniche, Y. S. Morita, C. M. Sassetti, E. H. M. Rego, M. S. Siegrist

This study investigates the role of RodA in mycobacterial cell wall synthesis under damage, showing its non-redundant function in spatially flexible peptidoglycan assembly and stress response.

bioRxiv · Oct 26, 2021 · PreprintMicrobiology

The mycobacterial cell wall partitions the plasma membrane to organize its own synthesis

A. Garcia-Heredia, T. Kado, C. E. Sein, J. Puffal, S. H. Osman, J. Judd, T. A. Gray, Y. S. Morita, M. S. Siegrist

The paper shows that in Mycobacterium smegmatis, peptidoglycan precursors are synthesized in distinct plasma membrane domains that are separate from sites of cell wall assembly, and this partitioning is essential for ordered synthesis.

bioRxiv · Jul 6, 2020 · PreprintMicrobiology

Trehalose recycling promotes energy-efficient mycomembrane reorganization in nutrient-limited mycobacteria

A. A. Pohane, C. R. Carr, J. Garhyan, B. M. Swarts, M. S. Siegrist

This study shows that trehalose recycling reduces energy costs for mycomembrane remodeling in nutrient-starved mycobacteria, enhancing survival under stress.

bioRxiv · Sep 4, 2019 · PreprintMicrobiology

Peptidoglycan precursor synthesis along the sidewall of pole-growing mycobacteria

A. Garcia-Heredia, A. A. Pohane, E. S. Melzer, C. R. Carr, T. J. Fiolek, S. R. Rundell, H. C. Lim, J. Wagner, Y. S. Morita, B. M. Swarts, M. S. Siegrist

The paper investigates peptidoglycan precursor synthesis in mycobacteria, showing that sidewall synthesis responds to cell wall damage while polar synthesis is linked to elongation.

bioRxiv · Jul 27, 2018Microbiology

DivIVA concentrates mycobacterial cell envelope assembly for initiation and stabilization of polar growth

E. S. Melzer, C. E. Sein, J. J. Chambers, M. S. Siegrist

This study shows DivIVA concentrates cell wall synthesis in mycobacteria to initiate and stabilize polar growth, supporting the idea that rod shape is a hardwired bacterial trait.

bioRxiv · Jun 7, 2018Microbiology