B. Seelbinder

Non-invasive Chromatin Deformation and Measurement of Differential Mechanical Properties in the Nucleus

This paper uses localized temperature gradients to measure nuclear mechanical properties, revealing distinct material responses in different compartments.

Epigenetic remodeling during monolayer cell expansion reduces therapeutic potential

This study shows that mechanical memory in chondrocytes is encoded in chromatin architecture, affecting their fate and therapeutic potential after expansion.

Dedifferentiation alters chondrocyte nuclear mechanics during in vitro culture and expansion

This study shows that dedifferentiated chondrocytes exhibit altered nuclear mechanics and gene expression of nuclear envelope proteins, which may contribute to further dedifferentiation.

Image-based elastography of heterochromatin and euchromatin domains in the deforming cell nucleus

This study develops nuclear elastography to measure the elasticity of heterochromatin and euchromatin in deforming cell nuclei, finding heterochromatin is four times stiffer than euchromatin under peak deformation.

TENSCell: A 3D-Printed Device for High-Magnification Imaging of Stretch-Activated Cells Reveals Divergent Nuclear Behavior to Different Levels of Strain

Intra-Nuclear Tensile Strain Mediates Reorganization of Epigenetically Marked Chromatin During Cardiac Development and Disease

The paper shows that intra-nuclear tensile strain influences H3K9me3 chromatin reorganization during cardiac development and disease.

Deformation microscopy for dynamic intracellular and intranuclear mapping of mechanics with high spatiotemporal resolution

This paper introduces deformation microscopy to quantify intracellular and intranuclear mechanics with high spatiotemporal resolution, revealing strain dynamics and structural heterogeneity in cells and nuclei.