Optimization of Golden Gate assembly through application of ligation sequence-dependent fidelity and bias profiling
Vladimir Potapov, Jennifer L. Ong, Rebecca B. Kucera, Bradley W. Langhorst, Katharina Bilotti, John M. Pryor, Eric J. Cantor, Barry Canton, Thomas F. Knight, Thomas C. Evans, Gregory J. S. Lohman
The paper develops a method to optimize Golden Gate assembly by profiling ligation fidelity and bias, enabling efficient assembly of up to 24 fragments in a single reaction.
ACS Synthetic Biology · Oct 18, 2018Peer-reviewedSynthetic Biology