Thomas C. Evans

RIMS-seq identifies methylase specificities and sequences bacterial genomes using a simple protocol, revealing new methylase targets in bacteria and synthetic communities.

EM-seq detects DNA methylation at single base resolution using less DNA than bisulfite sequencing, showing improved accuracy and reliability.

This paper introduces LR-EM-seq, an enzymatic deamination method for long-range methylation profiling of 5-mC and 5-hmC, achieving better-defined DMRs over 5 kb.

The paper develops a method to optimize Golden Gate assembly by profiling ligation fidelity and bias, enabling efficient assembly of up to 24 fragments in a single reaction.