Jennifer L. Ong

The paper develops a microdroplet-based functional genomic screening pipeline using DNA nanoflowers and cell-free expression to identify DNA-interacting enzymes.

The paper identifies 5mC-selective deaminases and demonstrates their use in ultra-sensitive methylome sequencing at single-base resolution.

This study evaluates the fidelity of uridine analog incorporation during in vitro transcription using different RNA polymerases and introduces a protocol to improve incorporation fidelity without affecting RNA yield.

The paper develops a method to optimize Golden Gate assembly by profiling ligation fidelity and bias, enabling efficient assembly of up to 24 fragments in a single reaction.