Bradley W. Langhorst

The paper describes integrating refgenie into Galaxy to manage and share reference datasets, with tools for retrieval and a global repository for easy access.

The paper evaluates the impact of the Omicron variant's N gene mutation on CDC N1 target detection and finds no significant reduction in assay performance.

The paper improves methylation calling by filtering reads in low-mappability regions, reducing mixed methylation calls in a clinical dataset.

EM-seq detects DNA methylation at single base resolution using less DNA than bisulfite sequencing, showing improved accuracy and reliability.

This study uses rapid diagnostics and metatranscriptomics to analyze SARS-CoV-2 infection, revealing viral evolution, host responses, and drug interactions in NYC samples.

The paper evaluates epigenetic methods using reference materials, finding high concordance between assays and differences in performance across platforms.

The paper develops a method to optimize Golden Gate assembly by profiling ligation fidelity and bias, enabling efficient assembly of up to 24 fragments in a single reaction.