Routine Single Particle CryoEM Sample and Grid Characterization by Tomography
A. J. Noble, V. P. Dandey, H. Wei, J. Brasch, J. Chase, P. Acharya, Y. Z. Tan, Z. Zhang, L. Y. Kim, G. Scapin, M. Rapp, E. T. Eng, W. J. Rice, A. Cheng, C. J. Negro, , B. Carragher
This study uses fiducial-less tomography to analyze cryoEM grids and finds that 90% of particles are adsorbed to air-water interfaces, impacting protein structure and single particle analysis.
bioRxiv · Dec 11, 2017Biophysics