A. des Georges

Membrane binding and pore formation is Ca2+-dependent for the Clostridioides difficile binary toxin

This study shows that Ca2+ dissociation from CDTb's RBD1 triggers pore formation in endosomal membranes, differing from other binary toxins that rely on pH changes.

Visualization of translation reorganization upon persistent collision stress in mammalian cells

The paper visualizes how translation reorganizes in mammalian cells under persistent collision stress, revealing changes in ribosome complexes and quality control mechanisms.

Structure of the cell-binding component of the Clostridium difficile binary toxin reveals a novel macromolecular assembly

This paper determines the atomic structures of the C. difficile binary toxin CDTb using cryoEM and X-ray crystallography, revealing novel di-heptamer forms and critical domains for toxicity and stability.

Functional Pathways of Biomolecules Retrieved from Single-particle Snapshots

The paper introduces a method to analyze conformational changes in biomolecules using single-particle cryo-EM data, revealing functional pathways in RyR1 that differ from discrete structure analyses.

ENRICH: a fast method to improve the quality of flexible macromolecular reconstructions

ENRICH improves 3D map quality from small, heterogeneous cryo-EM datasets by merging particles across conformations using optical flow.

Routine Single Particle CryoEM Sample and Grid Characterization by Tomography

This study uses fiducial-less tomography to analyze cryoEM grids and finds that 90% of particles are adsorbed to air-water interfaces, impacting protein structure and single particle analysis.

Conformational Dynamics and Energy Landscapes of Ligand Binding in RyR1

The paper uses cryo-EM data to analyze conformational dynamics and energy landscapes of ligand binding in RyR1, revealing multiple binding routes and structural changes.