Author

A. J. Noble

Listed by name: these papers carry no ORCID for this author, so different people with the same name may be mixed together.

Biology Disciplines

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Biophysics· 4 Biochemistry· 2 Cell Biology· 1 Microbiology· 1

Signature Assays, Organisms & Methods

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Assay:cryo-EM· 5 Approach:experimental assay· 5 Organism:human· 2 Approach:microscopy & imaging analysis· 2 Organism:bacteria (other)· 1 Research Focus:benchmarking· 1 Research Focus:cell type annotation· 1 Research Focus:chromatin & epigenetic regulation· 1

Frequent Co-Authors

E. T. Eng· 5 shared B. Carragher· 4 shared C. S. Potter· 4 shared A. Cheng· 3 shared H. Wei· 2 shared L. Y. Kim· 2 shared L. Y. Yen· 2 shared M. Kopylov· 2 shared

Activity by Year

2017-2022 · peak 2/yr

Co-Author Collaboration Network

10 top collaborators · 29 mutual co-author links

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5 shared papersBiophysics

E. T. Eng

Mutual co-authors in cluster

Author profile →

8 papers

Recent·Most cited

Fully Automated Multi-Grid Cryo-EM Screening using Smart Leginon

A. Cheng, P. Kim, H. Kuang, J. H. Mendez, E. Y. D. Chua, K. Maruthi, H. Wei, A. Sawh, M. F. Aragon, V. Serbynovskyi, K. Neselu, E. T. Eng, C. S. Potter, B. Carragher, T. Bepler, A. J. Noble

Smart Leginon automates cryo-EM grid screening, reducing operator time from 6 hours to <10 minutes and improving throughput.

bioRxiv · Jul 23, 2022 · PreprintBiophysics

Architecture of the human erythrocyte ankyrin-1 complex

F. Vallese, K. Kim, L. Y. Yen, J. D. Johnston, A. J. Noble, T. Cali, O. B. Clarke

This study presents structures of the human erythrocyte ankyrin-1 complex, revealing its architecture and interactions with membrane proteins.

bioRxiv · Feb 10, 2022Biochemistry

Heterogeneous non-canonical nucleosomes predominate in yeast cells in situ

Z. Y. Tan, S. Cai, A. J. Noble, J. K. Chen, J. Shi, L. Gan

This study uses cryo-ET to show that most nucleosomes in yeast cells are non-canonical, challenging previous assumptions about their structure.

bioRxiv · Apr 4, 2021Cell Biology

Waffle Method: A general and flexible approach for FIB-milling small and anisotropically oriented samples

K. Kelley, A. M. Raczkowski, O. Klykov, P. Jaroenlak, D. Bobe, M. Kopylov, E. T. Eng, G. Bhabha, C. S. Potter, B. Carragher, A. J. Noble

The paper introduces the Waffle Method to improve cryo-FIB/SEM workflows for preparing complex biological samples for cryo-ET, demonstrating its effectiveness with various samples and hardware.

bioRxiv · Oct 28, 2020Microbiology

Characterization of the SARS-CoV-2 S Protein: Biophysical, Biochemical, Structural, and Antigenic Analysis

N. G. Herrera, N. C. Morano, A. Celikgil, G. I. Georgiev, R. J. Malonis, J. H. Lee, K. Tong, O. Vergnolle, A. B. Massimi, L. Y. Yen, A. J. Noble, M. Kopylov, J. B. Bonanno, S. C. Garrett-Thomson, D. B. Hayes, , S. C. Almo

This study evaluates the production, characterization, and antigenicity of SARS-CoV-2 S protein constructs in two cell lines, showing high-quality yields and consistent behavior across assays.

bioRxiv · Jun 14, 2020Biochemistry

Eliminating effects of particle adsorption to the air/water interface in single-particle cryo-electron microscopy: Bacterial RNA polymerase and CHAPSO

J. Chen, A. J. Noble, J. Y. Kang, S. Darst

This study shows that CHAPSO detergent reduces orientation bias in bacterial transcription complexes by preventing particle adsorption at the air/water interface during cryo-EM.

bioRxiv · Oct 30, 2018Biophysics

Routine Determination of Ice Thickness for Cryo-EM Grids

W. J. Rice, A. Cheng, A. J. Noble, E. T. Eng, L. Y. Kim, B. Carragher, C. S. Potter

The paper presents two methods to measure ice thickness in cryo-EM grids during data collection to improve sample preparation and data quality.

bioRxiv · Apr 16, 2018Biophysics

Routine Single Particle CryoEM Sample and Grid Characterization by Tomography

A. J. Noble, V. P. Dandey, H. Wei, J. Brasch, J. Chase, P. Acharya, Y. Z. Tan, Z. Zhang, L. Y. Kim, G. Scapin, M. Rapp, E. T. Eng, W. J. Rice, A. Cheng, C. J. Negro, , B. Carragher

This study uses fiducial-less tomography to analyze cryoEM grids and finds that 90% of particles are adsorbed to air-water interfaces, impacting protein structure and single particle analysis.

bioRxiv · Dec 11, 2017Biophysics