Luciferase-LOV BRET enables versatile and specific transcriptional readout of cellular protein-protein interactions
C. K. Kim, K. F. Cho, M. W. Kim, A. Y. Ting
The paper introduces SPARK2, a transcriptional readout system for PPIs using luciferase-LOV BRET, enabling high-throughput screening and specific detection of interactions.
bioRxiv · Mar 18, 2019Bioengineering