A. Y. Ting

Cell type-specific intracellular protein delivery with inactivated botulinum toxin

This study demonstrates the use of inactivated botulinum toxin as a cell type-specific protein delivery platform, achieving selective cargo delivery to distinct cell populations.

Dynamic mapping of proteome trafficking within and between living cells by TransitID

TransitID maps proteome trafficking between cellular compartments and between cells with nanometer resolution, revealing new roles for stress granules and intercellular signaling.

Cilium proteomics reveals Numb as a positive regulator of the Hedgehog signaling pathway

This study identifies Numb as a ciliary protein that promotes Hedgehog signaling by facilitating Ptch1 exit from the cilium through endocytic mechanisms.

Proximity interactome analysis of Lassa polymerase reveals eRF3a/GSPT1 as a druggable target for host directed antivirals.

The paper identifies eRF3a/GSPT1 as a druggable host target for antiviral therapy against Lassa virus by analyzing its interactome using proximity proteomics.

A single-component luminescent biosensor for the SARS-CoV-2 spike protein

This paper develops a single-component luminescent biosensor for detecting SARS-CoV-2 spike protein using E. coli and S. cerevisiae, enabling detection with a cell phone camera.

Shared and distinctive neighborhoods of emerin and LBR revealed by proximity labeling and quantitative proteomics

The paper uses proximity labeling and proteomics to identify proteins interacting with emerin and LBR at the inner nuclear membrane, revealing shared and distinct partners.

A genetic model for in vivo proximity labeling of the mammalian secretome

This paper introduces a genetic mouse model for in vivo proximity labeling of the secretome, revealing organ-specific secretory profiles and serum trafficking.

A light-gated transcriptional recorder for detecting cell-cell contacts

This paper introduces TRACC, a light-gated transcriptional recorder that detects cell-cell contacts with high specificity and sensitivity in mammalian cells.

Functional interactomes of the Ebola virus polymerase identified by proximity proteomics in the context of viral replication

The paper identifies functional interactors of the Ebola virus polymerase using proximity proteomics and validates their role in promoting viral replication.

An engineered transcriptional reporter of protein localization identifies regulators of mitochondrial and ER membrane protein trafficking in high-throughput screens

The paper develops HiLITR, a tool to study protein trafficking by linking localization to reporter gene expression, and identifies genes affecting mitochondrial and ER membrane protein trafficking.

Proximity-labeling reveals novel host and parasite proteins at the Toxoplasma parasitophorous vacuole membrane

Proximity labeling at non-centrosomal microtubule-organizing centers reveals VAB-10B and WDR-62 as distinct microtubule regulators

The paper identifies VAB-10B and WDR-62 as distinct regulators of non-centrosomal microtubules using proximity labeling in C. elegans.

Split-TurboID enables contact-dependent proximity labeling in cells

Split-TurboID enables proximity labeling at organelle contact sites by splitting the TurboID enzyme and reconstituting it at these sites, identifying over 100 endogenous proteins.

Proteomics of protein trafficking by in vivo tissue-specific labeling

The paper develops an in vivo platform to study secreted protein trafficking between organs, identifies interorgan communication factors, and finds conserved adipokines affecting muscle activity.

LUZP1, a novel regulator of primary cilia and the actin cytoskeleton, is altered in Townes-Brocks Syndrome

The paper identifies LUZP1 as a regulator of primary cilia and actin cytoskeleton, showing its role in Townes-Brocks Syndrome through interactions with SALL1 and effects on ciliogenesis and Sonic Hedgehog signaling.

Atlas of Subcellular RNA Localization Revealed by APEX-seq

APEX-seq maps subcellular RNA localization with nanometer resolution, revealing nuclear transcriptome organization and mitochondrial mRNA pathways.

Application of TurboID-mediated proximity labeling for mapping a GSK3 kinase signaling network in Arabidopsis

This study uses TurboID-mediated proximity labeling to map the GSK3 kinase signaling network in Arabidopsis, identifying hundreds of BIN2-interacting proteins.

Proximity labeling of protein complexes and cell type-specific organellar proteomes in Arabidopsis enabled by TurboID

This study uses TurboID to identify protein interactions and cell type-specific organellar proteomes in Arabidopsis, revealing novel interactors of a transcription factor and subcellular proteomes.

Luciferase-LOV BRET enables versatile and specific transcriptional readout of cellular protein-protein interactions

The paper introduces SPARK2, a transcriptional readout system for PPIs using luciferase-LOV BRET, enabling high-throughput screening and specific detection of interactions.

Directed evolution of TurboID for efficient proximity labeling in living cells and organisms

The paper develops TurboID and miniTurbo, biotin ligase mutants with enhanced catalytic efficiency for proximity labeling in cells and organisms, significantly reducing labeling time and toxicity.

Live-cell mapping of organelle-associated RNAs via proximity biotinylation combined with protein-RNA crosslinking

This paper introduces APEX-RIP, a method to map RNA localization in subcellular compartments using proximity biotinylation and RNA-protein crosslinking, showing improved specificity and coverage over conventional methods.