Use of a ubiquitous gene-editing tool in budding yeast causes off-target repression of neighboring gene protein synthesis
Emily Nicole Powers, Charlene Chan, Ella Doron‐Mandel, Lidia Llacsahuanga Allcca, Jenny Kim Kim, Marko Jovanović, Gloria Ann Brar
The paper shows that deleting DBP1 in yeast using a selection cassette causes off-target repression of MRP51 through a LUTI-based mechanism, leading to mutant phenotypes.
eLife · Nov 30, 2022Peer-reviewedGenetics