Single-molecule assay reveals the impact of composition, RNA duplex, and inhibitors on the binding dynamics of SARS-CoV-2 polymerase complex
Terri C. Lovell, Heidi A. F. Dewling, Cynthia X. Li, Hery W. Lee, Calvin J. Gordon, Dana Kocíncová, Maulik D. Badmalia, Egor P. Tchesnokov, Matthias Götte, Gonzalo Cosa
This study uses a single-molecule assay to analyze the binding dynamics of SARS-CoV-2 polymerase complex subunits with RNA, revealing differences in binding stability between co-expressed and reconstituted complexes and the effects of inhibitors.
bioRxiv · Jan 10, 2025 · PreprintBiophysics