Hery W. Lee

The paper shows that the SARS-CoV-2 polymerase core RTC undergoes a conformational change after RNA binding to become elongation-competent, which is essential for viral RNA synthesis.

This study uses a single-molecule assay to analyze the binding dynamics of SARS-CoV-2 polymerase complex subunits with RNA, revealing differences in binding stability between co-expressed and reconstituted complexes and the effects of inhibitors.