D. O. Morgan

Phosphate-binding pocket on cyclin B governs CDK substrate phosphorylation and mitotic timing

The paper shows that a phosphate-binding pocket on cyclin B influences CDK substrate phosphorylation and mitotic timing in budding yeast.

Assembly of SARS-CoV-2 ribonucleosomes by truncated N* variant of the nucleocapsid protein

This study shows that the truncated N* variant of SARS-CoV-2 nucleocapsid protein can assemble into vRNPs similar to full-length N, requiring the leucine-rich helix and inhibited by a phosphomimetic SR region.

Reconstitution of the SARS-CoV-2 ribonucleosome provides insights into genomic RNA packaging and regulation by phosphorylation

The paper reconstitutes the SARS-CoV-2 ribonucleosome in vitro and shows phosphorylation of the N protein disrupts vRNP assembly.

Single-molecule analysis of specificity and multivalency in binding of short linear substrate motifs to the APC/C

The paper develops a single-molecule assay to study SLiM binding to APC/C and finds distinct affinities and multivalency in substrate recognition.

Multisite phosphorylation by Cdk1 initiates delayed negative feedback to control mitotic transcription

The paper shows that multisite phosphorylation of Ndd1 by Cdk1 initiates delayed negative feedback, controlling mitotic transcription through a pulse of gene expression.

Phosphorylation modulates liquid-liquid phase separation of the SARS-CoV-2 N protein

This study shows phosphorylation of SARS-CoV-2 N protein modulates its phase separation behavior, forming liquid-like droplets that may support viral genome processing.

Polyanions provide selective control of APC/C interactions with the activator subunit

The paper identifies polyanions as regulators of APC/C-activator interactions, showing they enhance dissociation and inhibit ubiquitination of low-affinity substrates while being blocked by high-affinity substrates.