A lipid nanoparticle platform for high yield CRISPR-targeted homology directed repair enables fully non-viral CAR T cell generation
Joy J. Chen, Atip Lawanprasert, A.L.W. Kleinhenz, Mehar G. D. Namala, Daniel Chu, York Tang, Valentine Lindarto, Michael Launspach, Danna Lee, Hao Wu, Niren Murthy, David N. Nguyen
This paper develops a lipid nanoparticle (LNP) platform for non-viral CRISPR-mediated homology directed repair (HDR) to generate CAR T cells with improved efficiency and reduced toxicity compared to electroporation.
bioRxiv · Aug 5, 2026 · PreprintBioengineering